MICE EXPRESSING LIMITED REPERTOIRE OF IMMUNOGLOBULIN LIGHT CHAINS Russian patent published in 2024 - IPC A01K67/27 C07K16/46 C12N15/85 

Abstract RU 2812814 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of obtaining the sequence of the variable domain of a human immunoglobulin heavy chain, a method of producing a fully human heavy chain of immunoglobulin, as well as a method of producing an antibody. These methods are as follows: immunizing a genetically modified mouse with an antigen of interest, where the mouse contains exactly two unrearranged human V immunoglobulin gene segmentsκ and five unrearranged human J immunoglobulin gene segmentsκ operably linked to the mouse immunoglobulin light chain constant region sequence at the endogenous mouse kappa light chain loci, as well as one or more unrearranged human immunoglobulinH gene segments, one or more unrearranged human immunoglobulinH gene segments, and one or more unrearranged human immunoglobulin gene segments human immunoglobulinH segments operably linked to the mouse immunoglobulin heavy chain constant region sequence at endogenous mouse heavy chain loci.

EFFECT: invention is effective for producing fully human antibodies.

22 cl, 19 dwg, 26 tbl, 14 ex

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Authors

Bebb, Robert

Makvirter, Dzhon

Makdonald, Linn

Stivens, Shon

Devis, Samyuel

Bakler, Devid R.

Meer, Karolina A.

Merfi, Endryu Dzh.

Dates

2024-02-02Published

2014-03-13Filed