FIELD: chemistry of polysaccharides.
SUBSTANCE: invention relates to the chemistry of polysaccharides. Disclosed is a bioactive composition based on a cross-linked hyaluronic acid (HA) salt containing resveratrol. The average molecular weight of hyaluronic acid is 1800 kDa. Resveratrol in the composition is chemically bound with hyaluronic acid at the same time being a bifunctional cross-linking agent for it. The composition is a dry powdery substance with the following ratio of components, wt%: resveratrol - 0.5-1.5; hyaluronic acid - 98.5-99.5. Also disclosed is a method for producing a bioactive composition based on a cross-linked hyaluronic acid salt. The method involves mixing powdered hyaluronic acid with an average molecular weight of 1800 kDa with resveratrol powder for homogenizing the mixture in a mill at 15-40°C for 10-15 minutes and mechanochemical processing by feeding the resulting homogeneous mixture of powders into a mechanochemical reactor such as a twin-screw extruder with sequential automatic moving to the feeding zone and the second and third zones of mechanochemical treatment with the ratio of the lengths of the zones, respectively,%: 20:40:40. Mechanochemical treatment is carried out with the pressure within 500-600 MPa and shear deformation of cam mechanisms with a shear angle of 180° in total for 2-4 minutes in a nitrogen flow at a temperature of 5-10°C in the feed zone, at a temperature of 90-100°С in the second mechanochemical zone and at a temperature of 5-15°С in the third mechanochemical zone.
EFFECT: invention is aimed at obtaining a composition in which the macromolecular chains of HA are cross-linked with resveratrol as well as at the development of an environmentally friendly method for obtaining the composition, which makes it possible to obtain previously unknown cross-linked HA salts chemically modified with resveratrol in a one-stage technological mode in the absence of a liquid medium without high energy labor and water consumption while receiving target products with a high yield.
2 cl, 3 dwg, 4 ex
