DEVICE AND METHOD FOR MAGNETIC FABRICATION Russian patent published in 2021 - IPC A61F2/02 

FIELD: biotech.

SUBSTANCE: invention relates to tissue engineering and space biomedicine. The method of biofabrication of a three-dimensional construct from a biomaterial in outer space is as follows. The biomaterial enclosed in a non-adhesive thermo-reversible biocompatible hydrogel inside the biofabrication chamber is activated by cooling it to the sol-gel transition temperature. The nutrient medium containing paramagnetic salts is displaced into the biofabrication chamber. The biofabrication chambers are placed in the magnetic system of the bioprinter for the magnetic levitation assembly so that the working volume of the said chamber is in the center of a non-uniform magnetic field created by the magnetic system of the bioprinter. Magnetic levitation assembly is carried out in the central region of a non-uniform magnetic field with the lowest field strength parameters from a biomaterial, randomly distributed in the working volume of the biofabrication chamber, dissolved in a nutrient medium with paramagnetic properties. The obtained three-dimensional construct is fixed by displacing the fixing solution into the biofabrication chamber. Tissue spheroids or cells are used as biomaterials. A cuvette for biofabrication of a three-dimensional construct from a biomaterial in outer space contains a biofabrication chamber, at least one container for a fixing solution, at least one container for a nutrient medium with paramagnetic properties. Each of the containers has a piston for displacing the fixing solution or nutrient medium with paramagnetic properties and is connected to the biofabrication chamber through a channel with a valve assembly for displacing the fixing solution or nutrient medium with paramagnetic properties into the biofabrication chamber. The containers and the biofabrication chamber are made with the possibility of being hermetically sealed. A bioprinter for fabricating a three-dimensional construct from a biomaterial in outer space includes a base made semicircular in section, in which at least one magnetic system is radially located, which consists of at least two neodymium ring magnets connected by the same poles, lateral, front and rear protective shields located on all sides of the bioprinter to shield the magnetic field. On the outer surface of the base there is at least one portal with a diaphragm fixed on it for installing the cuvette into the magnetic system so that the working volume of the said chamber is in the center of a non-uniform magnetic field created by the magnetic system. The use of a cuvette for the delivery of biomaterials into outer space, as well as for fixation and return of the obtained three-dimensional construct to the Earth.

EFFECT: group of inventions allows for frameless biofabrication of tissue engineering constructs using magnetic fields as a technological alternative to traditional approaches based on scaffolds in tissue engineering, the obtained three-dimensional tissue constructs have good viability and a high degree of fusion of spheroids, the development of a magnetic bioprinter design and a cuvette that would provide the possibility of loading biomaterials could be used to deliver them to the ISS and / or outer space, and return the received materials to Earth.

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