FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, and is a method for the production of allulose, providing following stages: conversion of polymer glucose carbohydrate into glucose-1-phosphate (hereinafter – G1P), using α-glucan or cellodextrin phosphorylase; conversion of G1P into glucose-6-phosphate (hereinafter – G6P), using phosphoglucomutase; conversion of G6P into fructose-6-phosphate (hereinafter – F6P), using phosphoglucoisomerase; conversion of F6P into alluloso-6-phosphate (hereinafter – A6P), using alluloso-6-phosphatepimerase (hereinafter – A6PE); and conversion of A6P into allulose, using alluloso-6-phosphatephosphatase (hereinafter – A6PP); where A6PP is A6PP Ruminiclostridium thermocellum, or where A6PE is A6PE Brevibacillus thermoruber.
EFFECT: invention allows for the production of allulose with high degree of efficiency.
15 cl, 8 dwg, 3 tbl, 14 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NOVEL THERMALLY STABLE FRUCTOSE-6-PHOSPHATE-3-EPIMERASE AND A METHOD OF PRODUCING ALLULOSE USING SAME | 2017 |
|
RU2733427C2 |
| ENZYMATIC PRODUCTION OF D-ALLULOSE | 2017 |
|
RU2819709C2 |
| RIBULOSE PHOSPHATE-3-EPIMERASE MOTIF WITH LOW SIDE REACTIVITY AND AN ENZYME CONTAINING THIS MOTIF | 2020 |
|
RU2792229C2 |
| NOVEL THERMOSTABLE TAGATOSE-6-PHOSPHATE PHOSPHATASE AND METHOD FOR PRODUCING TAGATOSE USING THEREOF | 2017 |
|
RU2722208C1 |
| ENZYMATIC PRODUCTION OF TAGATOSE | 2019 |
|
RU2820606C2 |
| ENZYMATIC PRODUCTION OF D-TAGATOSE | 2016 |
|
RU2749811C1 |
| ENZYMATIC HEXOSE PRODUCTION | 2018 |
|
RU2766710C2 |
| METHODS AND COMPOSITIONS FOR TREATING TUMORS | 2017 |
|
RU2741786C2 |
| METHOD FOR AMPLIFYING LOCI IN BACTERIAL CELL | 2009 |
|
RU2577985C2 |
| METHOD OF PREPARING GENETICALLY MODIFIED BACULOVIRUS, STRAIN OF NUCLEAR POLYHEDROSIS VIRUS, METHOD OF CONTROL OVER INSECTS, INSECTICIDE COMPOSITION | 1991 |
|
RU2099420C1 |
