FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, namely to a vaccine for immunisation for the purposes of protection against avian pathogens. The vaccine comprises one or several recombinant vectors of turkey herpesvirus (HVT). The vector comprises at least two heterologous polynucleotides. Each of the polynucleotides encodes and expresses an antigen of an avian pathogen. The heterologous polynucleotides are linked by IRES or P2A. The recombinant HVT vector for expression of antigens of avian pathogen comprises at least two heterologous polynucleotides, wherein each of the polynucleotides encodes and expresses an antigen of an avian pathogen. The two heterologous polynucleotides encode two antigens of an avian pathogen. The first heterologous polynucleotide comprises a polynucleotide encoding a polypeptide selected from the group consisting of a VP2 antigen of infectious bursal disease virus (IBDV), glycoprotein D antigen of infectious laryngotracheitis virus (ILTV), and F antigen of Newcastle disease virus (NDV-F). The second heterologous polynucleotide comprises a polynucleotide encoding a polypeptide selected from the group consisting of a VP2 antigen of infectious bursal disease virus (IBDV), glycoprotein D antigen of infectious laryngotracheitis virus (ILTV), and F antigen of Newcastle disease virus (NDV-F). Or the vector comprises heterologous polynucleotides encoding polypeptides selected from the group consisting of a VP2 antigen of infectious bursal disease virus (IBDV), glycoprotein D antigen of infectious laryngotracheitis virus (ILTV), and F antigen of Newcastle disease virus (NDV-F). The method for vaccinating or inducing an immune or protective response in an animal against one or several avian pathogens includes at least one administration of the vaccine or vector.
EFFECT: unexpectedly, vaccines containing the recombinant HVT vector were effective in protecting animals from various avian pathogens without interference.
25 cl, 38 tbl, 34 dwg, 30 ex
