FIELD: genetic engineering and biotechnology.
SUBSTANCE: invention relates to the field of genetic engineering and biotechnology, namely to guide RNAs of ribonucleoprotein complexes of the CRISPR-Cas system containing guide RNAs, and kits containing ribonucleoprotein complexes of the CRISPR-Cas system and specific oligonucleotides for preliminary amplification of a highly conserved region of the Staphylococcus aureus mecA antibiotic resistance gene.
EFFECT: invention makes it possible to effectively detect the Staphylococcus aureus mecA antibiotic resistance gene after specific amplification of this gene fragment; invention provides detection of single copies of Staphylococcus aureus mecA antibiotic resistance gene.
7 cl, 9 dwg, 6 tbl, 5 ex
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