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2 782 314

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IPC

C12N15/11(2006-01-01)

C12N15/113(2010-01-01)

C12N9/22(2006-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2021139120, 2021-12-27

(24)

Start date

2021-12-27

(22)

Actual filing date

2021-12-27

(45)

Published

2022-10-25

(72)

Inventor

Tiumentsev Aleksandr IgorevichTiumentseva Marina AlekseevnaPrelovskaia Anna NikolaevnaAkimkin Vasilii Gennadevich

(73)

Holder

Federalnoe Biudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatelskii Institut Epidemiologii» Federalnoi Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelei I Blagopoluchiia Cheloveka Tsnii Epidemiologii Rospotrebnadzora)

CRISPR-CAS12 SYSTEM FOR DETECTING THE MECA ANTIBIOTIC RESISTANCE GENE OF STAPHYLOCOCCUS AUREUS AT ULTRA-LOW CONCENTRATIONS Russian patent published in 2022 - IPC C12N15/11 C12N15/113 C12N9/22 C12Q1/6876

Abstract RU 2782314 C1

FIELD: genetic engineering and biotechnology.

SUBSTANCE: invention relates to the field of genetic engineering and biotechnology, namely to guide RNAs of ribonucleoprotein complexes of the CRISPR-Cas system containing guide RNAs, and kits containing ribonucleoprotein complexes of the CRISPR-Cas system and specific oligonucleotides for preliminary amplification of a highly conserved region of the Staphylococcus aureus mecA antibiotic resistance gene.

EFFECT: invention makes it possible to effectively detect the Staphylococcus aureus mecA antibiotic resistance gene after specific amplification of this gene fragment; invention provides detection of single copies of Staphylococcus aureus mecA antibiotic resistance gene.

7 cl, 9 dwg, 6 tbl, 5 ex

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RU 2 782 314 C1

Authors

Tiumentsev Aleksandr Igorevich

Tiumentseva Marina Alekseevna

Prelovskaia Anna Nikolaevna

Akimkin Vasilii Gennadevich

Dates

2022-10-25—Published

2021-12-27—Filed