METHOD FOR QUANTIFYING THE AMOUNT OF FLAVONOIDS IN THE BUDS OF QUERCUS ROBUR Russian patent published in 2022 - IPC A61K36/49 B01D11/02 G01N21/00 

FIELD: pharmaceuticals.

SUBSTANCE: invention relates to the chemical and pharmaceutical industry, namely, to a method for quantifying the amount of flavonoids in the buds of Quercus robur. Proposed is a method for quantifying the amount of flavonoids in the buds of Quercus robur, including a one-time extraction with ethyl alcohol of air-dried raw materials in a precisely weighed amount of 1 g, in the raw materials:extractant ratio of 1:50, followed by sample preparation and determination of the optical density by means of differential spectrophotometry, using a standard cinaroside sample or using the value of the theoretical specific absorption index in the absence thereof; wherein an aqueous-alcohol extraction is produced from the buds of Quercus robur by one-time extraction of the air-dried raw materials crushed to the size of particles passing through a sieve with holes of a 2 mm diameter for 120 minutes with 70% ethyl alcohol; the amount of flavonoids is quantified at a wavelength of 400 nm as calculated for cinaroside, and the content of the amount of flavonoids as calculated for cinaroside is calculated by the formula: wherein x is the content of the amount of flavonoids as calculated for cinaroside, %; D is the optical density of the tested solution; D_o is the optical density of the solution of the standard sample (CO) of cinaroside; m is the mass of the raw materials, g; m_o is the mass of cinaroside CO, g; W is the loss in mass during drying, %, and in the absence of a standard sample of cinaroside, the theoretical specific absorption index is used, equal to 334: where x is the content of the amount of flavonoids as calculated for cinaroside, %; D is the optical density of the tested solution; m is the mass of the raw materials, g; 334 is the specific absorption index (E) of cinaroside CO at 400 nm; and W is the loss in mass during drying, %.

EFFECT: above method is a specific and selective method for quantifying the amount of flavonoids in the buds of Quercus robur.

1 cl, 8 dwg, 1 ex