METHOD FOR QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN NIGERUS GRASS Russian patent published in 2022 - IPC A61K36/71 

FIELD: chemical and pharmaceutical industry.

SUBSTANCE: invention relates to the chemical and pharmaceutical industry and can be used in quality control centers for medicines and control and analytical laboratories in the quantitative determination of the amount of flavonoids in the (Nigella sativa L.) herb. Extraction is carried out with ethyl alcohol at a concentration of 70% of air-dry raw materials with an exact weight of 1 g, crushed to a particle size passing through a sieve with holes with a diameter of 2 mm, in the ratio "raw material-extractant" - 1:30 for 45 minutes. A water-alcohol extract is obtained from the herb Nigella sowing single extraction. Quantitative determination of the amount of flavonoids in the herb Nigella sativa is carried out at a wavelength of 412 nm in terms of rutin. The content of the sum of flavonoids in terms of rutin and absolutely dry raw materials is calculated by the formula

where X is the content of the sum of flavonoids in terms of rutin and absolutely dry raw materials,%; D is the optical density of the test solution; D_o is the optical density of a solution of a standard sample of rutin; m is the mass of raw materials, g; mo is the mass of the standard sample of rutin, g;

W - weight loss on drying, %; or according to the formula

where 231 is the specific absorbance () of a standard sample of rutin at 412 nm.

EFFECT: method allows to quantify the amount of flavonoids in the herb Nigella sativa in terms of rutin.

1 cl, 7 dwg, 2 ex