METHOD FOR QUANTITATIVE DETERMINATION OF THE SUM OF FLAVONOIDS IN POPLAR LEAVES IN BLACK Russian patent published in 2019 - IPC A61K36/76 A61K31/352 G01N33/15 

FIELD: medicine; pharmaceuticals.

SUBSTANCE: invention refers to chemical-pharmaceutical industry, namely to a method for quantitative determination of flavonoids in black poplar leaves. Method for quantitative determination of flavonoids in black poplar leaves, consisting in preliminary production of water-and-alcohol extraction from vegetable raw material by extraction of 1 g of a precise weighed portion of ground raw material ground to particle size of 1 mm with 70 % ethyl alcohol in terms of a substance of flavonoid nature, by differential spectrophotometry in relation "raw material-extractant" – 1:30, wherein flavonoids are determined at wavelength of 414 nm in terms of routine and absolutely dry material is calculated by formula

where x is content of sum of flavonoids in terms of rutin, %; D is the optical density of the test solution; D_o is the optical density of the solution of the State standard routine sample; m is weight of raw material, g; m_o is mass of State standard sample of rutin, g; W is weight loss during drying, %; in case of absence of standard routine sample, it is reasonable to use theoretical value of its specific absorption coefficient equal to 240,

where x is content of flavonoids sum in recalculation for routine, %; D is the optical density of the test solution; m is weight of raw material, g; 240 is specific absorption coefficient (E^1%_1cm) of the state standard routine at 414 nm; W is weight loss during drying, %.

EFFECT: method for quantitative determination of flavonoids in black poplar leaves is effective.

1 cl, 3 dwg, 3 tbl, 2 ex