FIELD: molecular biology.
SUBSTANCE: described is the use of a protein containing the amino acid sequence SEQ ID NO: 1, for non-specific splitting of single-stranded DNA molecules. Also described is a method of detecting nucleic acids of living organisms, involving the use of said protein.
EFFECT: creation of a new instrument for determining specific nucleic acid sequences based on collateral activity of ScCas12a nuclease.
5 cl, 5 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2791447C1 |
| DNA-CUTTING AGENT BASED ON Cas9 PROTEIN FROM THE BACTERIUM STREPTOCOCCUS UBERIS NCTC3858 | 2022 |
|
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| DNA-CUTTING AGENT BASED ON THE Cas9 PROTEIN FROM THE BACTERIUM CAPNOCYTOPHAGA OCHRACEA | 2021 |
|
RU2778156C1 |
| TOOL FOR CUTTING DOUBLE-STRANDED DNA USING CAS12D PROTEIN FROM KATANOBACTERIA AND HYBRID RNA PRODUCED BY FUSION OF GUIDE CRISPR RNA AND SCOUT RNA | 2020 |
|
RU2771626C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM DEMEQUINA SEDIMINICOLA BACTERIA | 2019 |
|
RU2722933C1 |
| NUCLEASE Cpf1 FROM BACTERIUM RUMINOCOCCUS BROMII, DNA MOLECULE OR RNA CODING NUCLEASE, VECTOR CONTAINING SAID DNA MOLECULE, CRISPR/Cpf1 SYSTEM CONTAINING SAID NUCLEASE AND GUIDE RNA, HOST CELL FOR PRODUCING NUCLEASE Cpf1, METHOD OF PRODUCING NUCLEASE Cpf1 AND USE THEREOF | 2022 |
|
RU2816876C1 |
| DNA PROTEASE CUTTING AGENT BASED ON Cas9 PROTEIN FROM DEFLUVIIMONAS SP. | 2018 |
|
RU2712492C1 |
| DNA POLYMER BASED ON Cas9 PROTEIN FROM BIOTECHNOLOGICALLY SIGNIFICANT BACTERIUM CLOSTRIDIUM CELLULOLYTICUM | 2018 |
|
RU2712497C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
|
RU2722934C1 |
| USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS | 2019 |
|
RU2724470C1 |
