FIELD: biotechnology.
SUBSTANCE: invention describes the use of the bacterial nuclease of the CRISPR-Cas12a system from the bacterium Sedimentisphaera cyanobacteriorum L21-RPul-D3 for the formation of highly specific double-strand breaks in the DNA molecule. This nuclease can be used as a tool to introduce changes in well-defined places in the genomic DNA sequence of unicellular or multicellular organisms.
EFFECT: increase in the versatility of available CRISPR systems is achieved, which will allow the use of Cas12a nucleases from various organisms to cut genomic or plasmid DNA at a greater number of specific sites and under different conditions.
5 cl, 1 tbl, 10 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MEANS FOR DETECTING NUCLEIC ACIDS BASED ON ScCas12a PROTEIN FROM SEDIMENTISPHAERA CYANOBACTERIORUM | 2023 |
|
RU2820345C1 |
| DNA-CUTTING AGENT BASED ON Cas9 PROTEIN FROM THE BACTERIUM STREPTOCOCCUS UBERIS NCTC3858 | 2022 |
|
RU2788197C1 |
| DNA POLYMER BASED ON Cas9 PROTEIN FROM BIOTECHNOLOGICALLY SIGNIFICANT BACTERIUM CLOSTRIDIUM CELLULOLYTICUM | 2018 |
|
RU2712497C1 |
| DNA PROTEASE CUTTING AGENT BASED ON Cas9 PROTEIN FROM DEFLUVIIMONAS SP. | 2018 |
|
RU2712492C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM DEMEQUINA SEDIMINICOLA BACTERIA | 2019 |
|
RU2722933C1 |
| USE OF CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA FOR MODIFYING GENOMIC DNA IN CELLS | 2019 |
|
RU2724470C1 |
| DNA PROTEASE CUTTING AGENT BASED ON CAS9 PROTEIN FROM PASTEURELLA PNEUMOTROPICA BACTERIA | 2019 |
|
RU2722934C1 |
| GENOMIC DNA EDITING SYSTEM OF AN EUKARYOTIC CELL BASED ON THE NUCLEOTIDE SEQUENCE ENCODING THE SUCAS9NLS PROTEIN | 2022 |
|
RU2804422C1 |
| DNA-CUTTING AGENT BASED ON THE Cas9 PROTEIN FROM THE BACTERIUM CAPNOCYTOPHAGA OCHRACEA | 2021 |
|
RU2778156C1 |
| TOOL FOR CUTTING DOUBLE-STRANDED DNA USING CAS12D PROTEIN FROM KATANOBACTERIA AND HYBRID RNA PRODUCED BY FUSION OF GUIDE CRISPR RNA AND SCOUT RNA | 2020 |
|
RU2771626C1 |
