FIELD: biotechnology.
SUBSTANCE: group of inventions relates to obtainment of nucleic acid libraries. A method for obtainment of a nucleic acid library includes obtainment of a set of single-stranded nucleic acids or a set of double-stranded nucleic acids with subsequent denaturation of the latter to obtain the set of single-stranded nucleic acids, and ligation of the first and the second adapters.
EFFECT: implementation of the group of inventions allows for simultaneous fragmentation of a genome and ligation of adapters to fragments, thereby simplifying obtainment of a nucleic acid library.
50 cl, 27 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MODULATION OF POLYMER GRANULES FOR DNA PROCESSING | 2019 |
|
RU2822154C2 |
| ANALYSIS OF MULTIPLE ANALYTES USING ONE ANALYSIS | 2019 |
|
RU2824049C2 |
| DIRECT CAPTURE, AMPLIFICATION AND SEQUENCING OF TARGET DNA USING IMMOBILISED PRIMERS | 2011 |
|
RU2565550C2 |
| NON-INVASIVE DIAGNOSIS BY SEQUENCING OF 5-HYDROXYMETHYLATED CELL-FREE DNA | 2017 |
|
RU2742355C2 |
| NUCLEIC ACID PROBE AND A METHOD OF DETECTING GENOME FRAGMENTS | 2014 |
|
RU2733888C2 |
| METHODS FOR ENCAPSULATING SINGLE CELLS, ENCAPSULATED CELLS, AND METHODS OF APPLICATION THEREOF | 2019 |
|
RU2750567C2 |
| METHODS AND SYSTEMS FOR OBTAINING SETS OF UNIQUE MOLECULAR INDICES WITH HETEROGENEOUS LENGTH OF MOLECULES AND CORRECTING ERRORS THEREIN | 2018 |
|
RU2766198C2 |
| LIBRARIES FOR NEXT GENERATION SEQUENCING | 2014 |
|
RU2698125C2 |
| COMPLEX SYSTEMS OF TRANSPOSOME BOUND ON SURFACE | 2020 |
|
RU2790295C2 |
| MULTIPLEX DETECTION OF NUCLEIC ACIDS | 2014 |
|
RU2733887C2 |
