FIELD: biotechnology.
SUBSTANCE: group of inventions relates to obtainment of nucleic acid libraries. A method for obtainment of a nucleic acid library includes obtainment of a set of single-stranded nucleic acids or a set of double-stranded nucleic acids with subsequent denaturation of the latter to obtain the set of single-stranded nucleic acids, and ligation of the first and the second adapters.
EFFECT: implementation of the group of inventions allows for simultaneous fragmentation of a genome and ligation of adapters to fragments, thereby simplifying obtainment of a nucleic acid library.
50 cl, 27 dwg, 4 ex
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|
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|
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|
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|
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|
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|
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|
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|
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| METHODS AND MEANS OF PRODUCING SEQUENCING LIBRARY | 2019 |
|
RU2815513C2 |
| TRANSPOSITION WITH PRESERVATION OF GENE ADHESION | 2015 |
|
RU2709655C2 |
