FIELD: medicine.
SUBSTANCE: invention relates to biotechnology and can be used in laboratory diagnostics of cytomegalovirus (CMV) drug resistance to ganciclovir (HCV) and valganciclovir (HCV) in patients with confirmed CMV infection.
EFFECT: in patients with confirmed CMV infection, a blood sample is taken in a standard way and the following is performed: isolation of CMV DNA; determination of CMV viral load; amplification of the UL97 gene fragment including codons 460, 520 and 590–607, using the following primers: acaacgtcacggtacatcga, gtcgtagtccaaactcgaga, cgacacgaggacatcttgg and various temperature regimes; detection and visualization of amplification products of the CMV UL97 gene fragment by evaluative electrophoresis; purification of amplification products; sequencing of the CMV UL97 fragment; analysis of the obtained results with determination of the genotype of the CMV UL97 gene fragment.
1 cl, 2 dwg, 2 ex
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