METHOD FOR MOLECULAR DIAGNOSTICS OF MUTATIONS IN THE UL97 GENE OF CYTOMEGALOVIRUS ASSOCIATED WITH DRUG RESISTANCE TO GANCICLOVIR AND VALGANCICLOVIR IN PATIENTS WITH CONFIRMED CYTOMEGALOVIRUS INFECTION Russian patent published in 2023 - IPC C12Q1/68 C12N1/00 

FIELD: medicine.

SUBSTANCE: invention relates to biotechnology and can be used in laboratory diagnostics of cytomegalovirus (CMV) drug resistance to ganciclovir (HCV) and valganciclovir (HCV) in patients with confirmed CMV infection.

EFFECT: in patients with confirmed CMV infection, a blood sample is taken in a standard way and the following is performed: isolation of CMV DNA; determination of CMV viral load; amplification of the UL97 gene fragment including codons 460, 520 and 590–607, using the following primers: acaacgtcacggtacatcga, gtcgtagtccaaactcgaga, cgacacgaggacatcttgg and various temperature regimes; detection and visualization of amplification products of the CMV UL97 gene fragment by evaluative electrophoresis; purification of amplification products; sequencing of the CMV UL97 fragment; analysis of the obtained results with determination of the genotype of the CMV UL97 gene fragment.

1 cl, 2 dwg, 2 ex