FIELD: medicine.
SUBSTANCE: invention relates to biotechnology and can be used in laboratory diagnostics of cytomegalovirus (CMV) drug resistance to ganciclovir (HCV) and valganciclovir (HCV) in patients with confirmed CMV infection.
EFFECT: in patients with confirmed CMV infection, a blood sample is taken in a standard way and the following is performed: isolation of CMV DNA; determination of CMV viral load; amplification of the UL97 gene fragment including codons 460, 520 and 590–607, using the following primers: acaacgtcacggtacatcga, gtcgtagtccaaactcgaga, cgacacgaggacatcttgg and various temperature regimes; detection and visualization of amplification products of the CMV UL97 gene fragment by evaluative electrophoresis; purification of amplification products; sequencing of the CMV UL97 fragment; analysis of the obtained results with determination of the genotype of the CMV UL97 gene fragment.
1 cl, 2 dwg, 2 ex
Title | Year | Author | Number |
---|---|---|---|
MOLECULAR DIAGNOSTIC TECHNIQUE FOR GENETIC RISK OF COMPLICATED UROGENITAL CHLAMYDIAL INFECTION CAUSING REPRODUCTIVE DYSFUNCTION IN INDIVIDUAL | 2012 |
|
RU2535724C2 |
METHOD FOR DIAGNOSING HELICOBACTER PYLORI INFECTION AND ITS RESISTANCE TO CLARITHROMYCIN AND LEVOFLOXACIN | 2022 |
|
RU2806581C1 |
METHOD OF DETECTING MUTATIONS IN COMPLEX DNA MIXTURES | 2014 |
|
RU2613489C2 |
DETERMINATION OF CANCER SUSCEPTIBILITY BY IDENTIFICATION OF GENOTYPIC COMBINATIONS OF SPECIFIC VERSIONS OF CYP1B1, BRCA2 AND CHEK2 GENES | 2006 |
|
RU2470998C2 |
METHOD FOR DIAGNOSING PORCINE ENZOOTIC PNEUMONIA BY POLYMERASE CHAIN REACTION | 2018 |
|
RU2702170C1 |
VTVAF17 GENE-THERAPY DNA VECTOR, METHOD FOR PRODUCING THEREOF, ESCHERICHIA COLI SCS110-AF STRAIN, METHOD FOR PRODUCING THEREOF, ESCHERICHIA COLI SCS110-AF/VTVAF17 STRAIN, BEARING VTVAF17 GENE-THERAPY DNA VECTOR, METHOD FOR PRODUCING THEREOF | 2017 |
|
RU2678756C1 |
SYNTHETIC OLIGONUCLEOTIDE-PRIMERS FOR DETECTION OF HUMAN CYTOMEGALOVIRUS DNA | 1999 |
|
RU2164945C1 |
OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR IDENTIFYING GENOME OF RABBIT VIRAL HEMORRHAGIC DISEASE BY REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION | 2009 |
|
RU2416648C1 |
METHOD AND KIT FOR DETECTING CAMPYLOBACTER BACTERIA IN PATIENTS WITH INFLAMMATORY INTESTINAL DISEASES | 2019 |
|
RU2732923C1 |
METHOD OF MICROORGANISM DETECTION IN BIOLOGICAL SAMPLE | 2007 |
|
RU2435865C2 |
Authors
Dates
2023-03-13—Published
2022-04-30—Filed