FIELD: cell biology; oncology.
SUBSTANCE: invention relates to experimental oncology, and is a method for selecting mitochondria for experimental mitochondrial therapy of melanoma B16-F10. To implement this method, first, heart mitochondria of intact rats are introduced into the B16-F10 melanoma cell culture. After that, with the degree of scratch overgrowth less than 25%, the average background value of the oxygen consumption rate — OCR between the control and the variant with the addition of mitochondria is at least 206.7 pmol/min, with a difference between the maximum values of at least 230.9 pmol/min, with a decrease the base level of glycolysis activity — ECAR not less than 14.36 mrn/min select mitochondria for further use in mitochondrial therapy of melanoma.
EFFECT: invention improves efficiency in the selection of mitochondria to enable mitochondrial therapy for melanoma B16-F10.
1 cl, 1 ex
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