METHOD FOR STUDYING THE CONTROL OF STAPHYLOCOCCUS AUREUS BIOFILMS WITH A MEDICINAL PRODUCT BASED ON SILVER NANOPARTICLES AND DIMETHYL SULFOXIDE Russian patent published in 2023 - IPC G01N33/00 A61K33/38 A61K31/10 A61L2/18 A61P31/04 A61L101/02 A61L101/40 

FIELD: medicine; veterinary medicine.

SUBSTANCE: invention can be used to assess the destruction of biofilms. A method for studying the fight against biofilms of Staphylococcus aureus with a drug based on silver nanoparticles and dimethyl sulfoxide consists in adding an equal volume of 0.2 ml of dimethyl sulfoxide with an active ingredient content of 1,000 mg/ml to 0.2 ml of a solution of the drug argovit with an active ingredient content of 13 mg/ml. ml, 0.2 ml meat-peptone broth and 0.2 ml 1.5×10⁶ CFU/ml of the reference strain of Staphylococcus aureus ATCC 25953 or isolate of Staphylococcus aureus isolated from cattle with a clinical manifestation of an infectious disease, followed by incubation for 24 hours at T=37.5±0.5°C. The result of the biofilm control study is determined by the change in the intensity of biofilm formation by measuring the optical density on a spectrophotometer at a wavelength of 492 nm.

EFFECT: invention allows evaluating the ability of a preparation based on silver and dimethyl sulfoxide nanoparticles to prevent the formation of Staphylococcus aureus biofilms.

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