FIELD: medicine; veterinary medicine.
SUBSTANCE: invention can be used for the destruction of biofilms. A method of studying the reduction of Staphylococcus aureus biofilm formation with a drug based on silver nanoparticles and dimethyl sulfoxide includes adding an equal volume of 0.2 ml of dimethyl sulfoxide with an active ingredient content of 0,2 mg/ml to 0.2 ml of a solution of argovit with an active ingredient content of 13 mg/ml, 0.2 ml of meat-peptone broth with 50 ml of active substance and 0.2 ml of 1.5×106 CFU/ml of the reference strain of Staphylococcus aureus ATCC 25953 or isolate of Staphylococcus aureus isolated from cattle with a clinical manifestation of an infectious disease, followed by incubation for 24 hours at T = 37.5±0.5°C. The result of the biofilm control study is determined by the change in the intensity of biofilm formation by measuring the optical density on a spectrophotometer at a wavelength of 492 nm.
EFFECT: invention makes it possible to study the reduction of biofilm formation by Staphylococcus aureus with a medicinal composition containing silver nanoparticles and ceftiofur.
1 cl, 1 tbl, 2 ex
