METHOD OF STUDYING REDUCTION OF ESCHERICHIA COLI BIOFILM FORMATION WITH MEDICINAL COMPOSITION CONTAINING SILVER NANOPARTICLES AND CIPROFLOXACIN Russian patent published in 2024 - IPC G01N33/48 C12Q1/18 

FIELD: medicine and veterinary medicine.

SUBSTANCE: following is proposed: a method of reducing the formation of biofilm of Escherichia coli with a therapeutic composition containing silver nanoparticles and enrofloxacin, the said method includes adding 0.2 ml of meat-peptone broth, 0.2 ml of enrofloxacin with a content of active ingredient 2 mg/ml and 0.2 ml of 1.5⋅10⁶ cfu/ml of the reference strain of Escherichia coli АТСС 25922 or isolate of Escherichia coli isolated from cattle with a clinical manifestation of an infectious disease, followed by incubation for 24 hours at T=37.5± 0.5°C, the result of the decrease in biofilm formation is determined by the change in the intensity of biofilm formation by measuring the optical density on a spectrophotometer at a wavelength of 492 nm.

EFFECT: invention makes it possible to study the reduction of biofilm formation by Escherichia coli.

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