METHOD OF INCREASING THE EFFICIENCY OF IN VITRO CULTIVATION OF COMMON BASIL (OCIMUM BASILICUM) CALLUS CULTURE Russian patent published in 2023 - IPC A01H4/00 

FIELD: biotechnology.

SUBSTANCE: invention is a method of increasing the efficiency of in vitro cultivation of Common Basil (Ocimum basilicum) callus culture, including sterilization of planting material, preparation of culture medium, cultivation under sterile conditions at a temperature of 25±2°C and air humidity of 60-70%, subcultivation of callus culture after 28 days, characterized in that it includes sterilization of Common Basil (Ocimum basilicum) lamina with 10% hydrogen peroxide solution for 5 minutes, washing three times with distilled water for 10 minutes, segmentation of Common Basil lamina into parts of 5x5 mm in size, cultivation on the culture medium of the following composition (wt.%): ammonium nitrate - 4.75, potassium nitrate - 5.47, calcium chloride 2-hydrate - 1.27, magnesium sulfate 7- hydrate - 1.06, potassium dihydroorthophosphate - 0.49, EDTA disodium salt - 0.1, iron sulfate 7-hydrate - 0.08, boric acid - 0.02, magnesium sulfate 4-hydrate - 0.06, zinc sulfate 7-hydrate - 0.02, potassium iodide - 0.002, sodium molybdate 2-hydrate - 0.001, copper sulfate 5-hydrate - 0.001, cobalt chloride 2-hydrate - 0.001, glycine - 0.01, mesoinositol - 0.3, nicotinic acid - 0.001; pyridoxine - 0.002; sucrose - 86.35; benzylaminopurine 0.01, naphthylacetic acid 0.003 under sterile conditions in climate chamber under continuous blue light with an intensity of 1500 lux of Quantum Line Samsung 281b LED in 24/24 h (light, dark) mode, subcultivation of Common Basil callus culture after 28 days and finally inoculation of the Common Basil callus culture with 10 µM salicylic acid (0.64 mg/l) on the same hormonal culture medium.

EFFECT: invention allows to obtain biomass of Schisandra chinensis callus culture with high yield and high content of biologically active substances of phenolic nature.

1 cl, 3 tbl