LACTASE ENZYMES WITH IMPROVED ACTIVITY AT LOW TEMPERATURES Russian patent published in 2023 - IPC A23C9/12 C12N9/38 A23C9/20 

FIELD: dairy industry and biotechnology.

SUBSTANCE: method of producing a dairy product includes the following: (a) mixing a milk-based substrate containing lactose at a concentration of at least 10 g/l and a peptide exhibiting beta-galactosidase activity at a concentration of 10 to 55 mg/l; (b) incubating the mixture at 1°C to 10°C for a period of time sufficient to reduce the concentration of lactose in the mixture to less than 2 g/l, where the pH in step (b) is from 4 to 7, and where the peptide, exhibiting beta-galactosidase activity, is a peptide having the amino acid sequence represented by the following SEQ ID NO: 12, 13, 14, 15, 16, 17, 18, 19, 25, 31, or enzymatically active fragments thereof, or the amino acid sequence of any of them having at least 90% sequence identity with the following SEQ ID NO: 12, 13,14, 15, 16, 17, 18, 19, 25 or 31. Also a method of containing lactose in a milk-based substrate using the said peptide exhibiting beta-galactosidase activity and its use in a method for producing a dairy product are described.

EFFECT: group of inventions makes it possible to effectively use enzymes with beta-galactosidase activity to reduce the lactose content in dairy products.

15 cl, 30 dwg, 3 tbl, 30 ex