FIELD: biotechnology.
SUBSTANCE: non-naturally occurring microorganism that synthesizes acetylneuraminic acid (Neu5Ac) has been proposed. Moreover, the specified microorganism has a pathway for the synthesis of sialic acid, including at least one heterologous enzyme, and the pathway for the biosynthesis of sialic acid includes glutamine-fructose-6-phosphate aminotransferase, glucosamine-6-phosphate-N-acetyltransferase, N-acetylglucosamine-2- epimerase, N-acetylneuraminic acid synthase and sugar phosphatase of the HAD-like phosphatase superfamily. Also in this microorganism, the natural catabolic pathway of sialic acid and the phosphoenolpyruvate: sugar-phosphotransferase system for importing saccharide, which is not used as a carbon source during the enzymatic production of Neu5Ac, are blocked, and the microorganism can use the exogenous carbon source present in the fermentation broth in as the sole carbon source without using the phosphoenolpyruvate:sugar phosphotransferase system to access this exogenous carbon source. The use of the specified microorganism for the production of Neu5Ac and a method for producing Neu5Ac using the specified microorganism are also proposed.
EFFECT: increased production of Neu5Ac.
11 cl, 4 dwg, 6 ex
