FIELD: biotechnology.
SUBSTANCE: invention concerns a new symbiotic mixture of bacteria that produces a xanthanase enzyme complex. The basis of the symbiotic mixture is made up of strains identified as a result of metagenomic analysis: Paenibacillus sp.FSL R7-277 — 83%, Cellulosimicrobium cellulans — 17%. Percentages reported at taxonomic levels are percentages for mapped reads only. Metagenomic reads have been deposited in the NCBI Sequence Read Archive (SRA) database (BioProject database number PRJNA849974). The composition of the proposed xanthanase complex was determined by measuring the specific activities of the following enzymes: 27.5 units/g of beta-glucosidase, 25.2 units/g of alpha-mannosidase, 6.3 units/g of beta-mannosidase, 7.9 units/g of endoglucanase, 183 units/g of xanthan lyase.
EFFECT: invention makes it possible to expand the range of microorganisms producing enzymes that degrade xanthan, and can also be used in biotechnology to produce new highly specific enzymes capable of complete destruction of the main and side chains of xanthan.
1 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| BIOCATALYST FOR THE DESTRUCTION OF XANTHAN BASED ON IMMOBILIZED CELLS OF SYMBIOTIC MIXTURE OF BACTERIA | 2022 |
|
RU2806184C1 |
| STRAIN OF PAENIBACILLUS TAICHUNGENSIS VKM B-3510D FOR XANTHAN DESTRUCTION | 2020 |
|
RU2754795C1 |
| STRAIN OF TRICHODERMA LONGIBRACHIATUM TW-14-220 FILAMENTOUS FUNGUS - PRODUCER OF CELLULASES, BETA-GLUCANASES AND XYLANASES FOR FEED PRODUCTION AND A METHOD FOR OBTAINING A FEED COMPLEX ENZYME PREPARATION | 2017 |
|
RU2654564C1 |
| COHNELLA SP. STRAIN 56 VKM B-3672D FOR DESTRUCTION OF XANTHAN | 2022 |
|
RU2805416C1 |
| TRICHODERMA REESEI MYCELIAL FUNGUS STRAIN - PRODUCER OF ENDOGLUCANASE, XYLANASE AND PECTINASE COMPLEX FOR PRODUCTION OF PROTEIN ADDITIVES BASED ON CEREAL AND LEGUME RAW MATERIAL FOR USE IN FODDER PRODUCTION | 2018 |
|
RU2696074C1 |
| NUCLEOTIDE SEQUENCE CODING LYTICASE ENZYME, AND PANEL OF OLIGONUCLEOTIDES FOR OBTAINING SYNTHETIC NUCLEOTIDE SEQUENCE OF LYTICASE GENE | 2023 |
|
RU2826150C1 |
| USE OF RECOMBINANT BETA-GALACTOSIDASE AsBgl 1390 FROM ARCHAEA SACCHAROVORANS AS BETA-GLUCOSIDASE, BETA-XYLOSIDASE AND BETA-MANNOSIDASE | 2012 |
|
RU2523581C2 |
| COMPOSITION FOR PRODUCTION OF HIGH-QUALITY FODDER OF PERENNIAL HIGH-PROTEIN LEGUMES | 2018 |
|
RU2706068C2 |
| STRAIN OF BACTERIA PAENIBACILLUS SPECIES - A PRODUCER OF β-GLUCANASE | 2017 |
|
RU2673967C1 |
| GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE | 2013 |
|
RU2550044C2 |
