FIELD: biotechnology.
SUBSTANCE: method for producing an antibody-ligand conjugate, in which the ligand is a molecule capable of binding to the ASGPR receptor. The method includes the stages of ligand activation in the process of sequential interaction with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysulfosuccinimide in phosphate or phosphate-buffered saline at pH 5.0-7.0 with the formation of N-hydroxysulfosuccinimide ester of the ligand and bioconjugation, during which the N-hydroxysulfosuccinimide ester of the ligand interacts with the primary amino group of the antibody in phosphate or phosphate-buffered saline at pH 7.2-7.4.
EFFECT: expanding the range of bioconjugation methods.
5 cl, 7 dwg, 3 tbl, 3 ex
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