FIELD: microbiology.
SUBSTANCE: invention relates to a method for obtaining a target molecule by converting a carbon source during an enzymatic process. The proposed method includes the cultivation of a microorganism genetically modified for the production of the target molecule in a suitable culture medium containing carbohydrate as a carbon source, and the isolation of the target molecule from the culture medium. The specified genetically modified microorganism includes functional genes encoding a phosphotransferase system (hereinafter – PTS) for carbohydrate utilization, where the specified carbohydrate is transported by PTS system for carbohydrate utilization. At the same time, ppsR gene encoding a protein of bifunctional ADP-dependent kinase-phosphate-dependent pyrophosphorylase DUF299 is deleted in the specified genetically modified microorganism.
EFFECT: invention provides an increase in the production of the target molecule obtained during fermentation in a microorganism.
10 cl, 4 tbl, 4 ex
| Title |
Year |
Author |
Number |
| ENZYMATIVE SYNTHESIS OF N-ACETYLNEURAMIC ACID |
2018 |
- Jennevajn, Shtefan
- Vartenberg, Dirk
|
RU2809787C2 |
| ENZYMATIC PRODUCTION OF SIALYLATED SACCHARIDES |
2019 |
- Jennevajn, Shtefan
- Vartenberg, Dirk
|
RU2819876C2 |
| MICROORGANISM FOR PRODUCING MYCOSPORINE-LIKE AMINO ACID AND A METHOD OF OBTAINING MYCOSPORINE-LIKE AMINO ACID USING IT |
2018 |
- Kim Sol
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- Lee Joo Hee
- Seok Jong-Cheol
- Jang Jae Woo
|
RU2736362C1 |
| MICROORGANISM PRODUCING MYCOSPORINE-LIKE AMINO ACID AND METHOD FOR PRODUCING MYCOSPORINE-LIKE AMINO ACID USING SAID MICROORGANISM |
2019 |
- Kim Sol
- Seok Jong-Cheol
- Lee Kyusung
- Jang Jae Woo
|
RU2757424C1 |
| RECOMBINANT STRAIN BASED ON ESCHERICHIA COLI, A METHOD OF ITS CONSTRUCTION AND USE |
2020 |
- Vej, Ajin
- Men, Gan
- Tszya, Khuejpin
- Gao, Syaokhan
- Ma, Fenyun
- Chzhou, Syaotsyun
- Chzhao, Chunguan
- Yan, Lipen
- Su, Khoubo
|
RU2813283C2 |
| METHOD FOR REDUCING ERRONEOUS INCLUSION OF NON-CANONICAL BRANCHED-CHAIN AMINO ACIDS |
2019 |
- Hauptmann, Peter
- Corcoles Garcia, Angel
- Lattemann, Claus Tobias
- Matzen, Arne
- Neubauer, Peter
|
RU2816654C2 |
| ESCHERICHIA COLI BL21(DE3)PLYSS/PET15B-HISCPF1 STRAIN: PRODUCER OF RNA-GUIDED ENDONUCLEASE CRISPR/CPF1 |
2021 |
- Vasikhovskaya Valeriya Aleksandrovna
- Romanenko Margarita Vladimirovna
- Netesov Sergej Viktorovich
|
RU2774120C1 |
| BACTERIUM WHICH PRODUCES MONOPHOSPHORYL LIPID A, AND A METHOD OF PRODUCING MONOPHOSPHORYL LIPID USING A BACTERIUM |
2016 |
- Chung Hak Suk
- Yang Eun Gyeong
- Hwang Dohyeon
|
RU2727014C2 |
| NEW VARIANT OF AN L-TRYPTOPHAN EXPORTING PROTEIN AND METHOD FOR PRODUCING L-TRYPTOPHAN WITH APPLICATION THEREOF |
2020 |
- Jung Moo Young
- Kim Hyun Ah
- Seo Chang Il
- Lee Imsang
- Kim Ji-Won
- Kim Tae Yeon
- Son Sung Kwang
- Cheong Ki Yong
|
RU2763603C1 |
| RECOVERED ANTIBODY (VERSIONS), A METHOD FOR PRODUCING THE ANTIBODY, AN ISOLATED NUCLEIC ACID, AN EXPRESSION CASSETTE (VERSIONS), A PLASMID (VERSIONS), A HOST CELL, A PHARMACEUTICAL PREPARATION, A KIT, A METHOD OF TREATING A PATIENT, AT RISK OF OR SUFFERING FROM ESCHERICHIA COLI INFECTION, AND A DIAGNOSTIC TECHNIQUE FOR DETERMINING ESCHERICHIA COLI INFECTIONS |
2014 |
- Vaskes Maksimiliano
- Gvchalya Luis
- Nad Gabor
- Nad Ester
- Sijyarto Valeriya
|
RU2724530C2 |
