IN VIVO METHOD FOR DETERMINING BIOCOMPATIBILITY OF SCAFFOLDS FOR NEUROTRANSPLANTATION Russian patent published in 2024 - IPC A61B6/00 

FIELD: medicine; physiology; neurobiology.

SUBSTANCE: invention can be used to determine the biocompatibility of scaffolds for neurotransplantation. A traumatic brain injury is created in the animal, the damaged area is removed, and a scaffold is implanted in the area of the injury. Determination of the neurological status of animals, intravital visualization of the dynamics of morphological changes in brain tissue in the area of implantation, assessment of the general motor and orientational-exploratory activity of the animal using the Open Field test, assessment of short-term and/or long-term memory in the post-traumatic period using the passive avoidance conditioned reflex test and the Morris water maze test, assessment of morphometric parameters of histological examination of brain tissue in the implantation zone. The biocompatibility of the scaffold is determined by examining the neurological deficit of the animal at least 2 months after implantation. A positive result of biocompatibility is achieved with the simultaneous fulfilment of several conditions: preservation of the general motor and orientation-exploratory activity of animals in the post-traumatic period; preservation of animals' abilities to learn and remember information; reduction in the focus of inflammation in the brain of animals relative to the control by 1.2 times.

EFFECT: method provides an assessment of the risks of developing pathological reactions that can lead to rejection of the transplanted material by nervous tissue, due to the stated conditions.

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