FIELD: biotechnology.
SUBSTANCE: disclosed is a method of producing recombinant hyaluronidase PH20 or a variant thereof. Method makes it possible to change the levels of N-glycans under cultivation conditions, including controlled concentration of glucose in the culture medium and low temperature of cultivation during a certain period of cultivation.
EFFECT: invention provides obtaining recombinant hyaluronidase PH20 or its variant with increased specific activity and productivity.
13 cl, 20 dwg, 4 tbl, 11 ex
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