FIELD: biotechnology.
SUBSTANCE: invention relates to a method for diagnosing predisposition of Duroc pigs to bursitis formation. Said method involves DNA extraction, amplification of a ROBO2 gene fragment containing point g.178817874, SSC13, Sscrofa11.1, using primers rs81327419-F and rs81327419-R, restriction of the amplified fragment with the restriction endonuclease BsrI and determination of the undesirable genotype GG\ROBO2 based on the obtained electrophoregram.
EFFECT: invention provides diagnosing predisposition of pigs of Duroc breed to formation of bursitis, based on determination of genetic variants of SNP rs81327419 (SSC13, Sscrofa11.1), caused by nucleotide substitution A→G at point g.178817874, for the purpose of typing breeding pigs for the carrier of harmful allelic variants.
1 cl, 1 dwg, 1 tbl, 1 ex
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