BIOSENSOR ANALYTICAL DEVICE FOR DETECTING LEVEL OF WATER CONTAMINATION WITH BIODEGRADABLE ORGANIC COMPOUNDS Russian patent published in 2024 - IPC C12M1/42 C12Q1/02 

FIELD: ecology; measurement; biotechnology.

SUBSTANCE: invention relates to ecology, specifically to biosensor analytical devices. Claimed device can be used to detect (determine) the level of water contamination with biodegradable organic compounds based on measuring biochemical oxygen consumption in water samples of various origins, including surface fresh, underground (ground), drinking, waste and treated waste water. Biosensor analytical device for detecting the level of contamination of water with biodegradable organic compounds comprises measuring cell (3) with a buffer solution, magnetic stirrer (4) and a biosensor system comprising oxygen electrode (1) configured to be connected to a device recording the response of the biosensor system, and receptor element (2) placed on oxygen electrode (1), comprising an association of microorganisms immobilized on a carrier. Microorganisms used are Paracoccus yeei VKM B-3302, Pseudomonas veronii VKM B-3835 and Rhodococcus fascians VKM Ac-2996 in weight ratio of 1:1:1, immobilized on nitrocellulose membrane and treated from above with a solution of chitosan, which forms a hydrogel after drying.

EFFECT: increasing the upper limit of the range of determined values of biochemical oxygen consumption (BOC) to 200 mg/dm³, increasing the operational stability of the response of the biosensor system, as well as increasing the time of stable operation of the biosensor system.

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