FIELD: biotechnology.
SUBSTANCE: invention relates to a method for determining concentration of an analyte in a sample of physiological fluid using mobile device (112) having at least one chamber (120), wherein the method involves: i) obtaining, by camera (120), at least one image of at least part of the reference colour map (118) and at least part of at least one test field (116) with a reagent of at least one optical test strip (114) with a sample applied thereon, wherein on the image, test field (116) is in a certain position relative to the reference colour card (118); reference colour map (118) comprises a plurality of different reference grey fields (126), locally associated with test field (116), wherein the plurality of reference grey fields (126) and test field (116) are locally related to each other, being arranged in adjacent positions, or plurality of reference grey fields (126), locally related to test field (116), are located on reference colour map (118) so that plurality of fields (126) of reference grey surrounds test field (116), and also reference colour map (118) comprises a plurality of different reference colour fields (128) having known reference colour values, and a plurality of different reference grey fields (126), locally associated with reference colour fields (128), wherein multiple fields (126) of reference grey and fields (128) of reference colours are locally related to each other by arrangement in adjacent positions or a plurality of reference grey fields (126), locally associated with reference colour fields (128), are located on a reference colour map (118) such that a plurality of reference grey fields (126) surround reference colour fields (128); ii) superimposing at least one predetermined mid-tone map correction based on pixels on the image obtained in step i), thereby obtaining at least one intensity-corrected first image, wherein the predetermined pixel-based mid-tone map correction includes associating a second luminance value with a first luminance value, wherein the first luminance value is recorded by camera (120), wherein each pixel of the recorded image is individually corrected by means of a predetermined correction of the mid tone map based on the pixels, wherein the mid tone map correction is obtained by combining a plurality of tone map corrections for different types of mobile devices; iii) obtaining from the first image with intensity correction information (174) on local brightness for at least some fields (128) of reference colours and for test field (116), using fields (126) of reference grey, locally associated with fields (128) of reference colours and test field (116), respectively, wherein the local brightness information (174) comprises a numerical index describing the local intensity of at least one colour of RGB fields (128) of the reference colours and test field (116), respectively; iv) superimposing at least one mobile device-specific tone map on a first intensity-corrected image, wherein the mobile device-specific tone map correction takes into account local brightness information (174), thereby obtaining at least one second intensity-corrected image; v) determining the analyte concentration based on the colour formation reaction of test field (116) using a second intensity-corrected image.
EFFECT: enabling convenient determination of an analyte in a physiological fluid sample using a mobile device with high accuracy and reliability of analytical measurement, taking into account local variations and device-specific aspects.
14 cl, 9 dwg
