FIELD: biotechnology.
SUBSTANCE: group of inventions relates to a method of producing a population of insulin-producing cells or a population β-cells of pancreas, having reduced number of Ki67-positive cells. Method comprises treating a population of endocrine precursor cells or cells at a later stage of differentiation with an FGFR1 inhibitor, which is 1-[2-amino-6-(3,5-dimethoxyphenyl)pyrido(2,3-d) pyrimidin-7-yl]-3-tert-butylurea, E-3810, PD-173074 or its salt. Produced cell population contains Ki67-positive cells in amount of less than 3%.
EFFECT: obtained cells can be used as a cell drug for treating diabetes mellitus.
12 cl, 1 dwg, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING BIOLOGICAL TISSUE-LIKE STRUCTURE | 2020 |
|
RU2815583C2 |
| IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO PANCREATIC ENDODERMAL CELLS (PEC) AND ENDOCRINE CELLS | 2020 |
|
RU2764465C1 |
| CELL COMPOSITIONS DERIVED FROM DEDIFFERENTIATED REPROGRAMMED CELLS | 2014 |
|
RU2675928C2 |
| IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN PANCREATIC ENDODERM CELLS (PEC) AND ENDOCRINE CELLS | 2014 |
|
RU2670064C2 |
| IN VITRO DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO PANCREATIC ENDODERMAL CELLS (PEC) AND ENDOCRINE CELLS | 2014 |
|
RU2734657C2 |
| CELL COMPOSITIONS OBTAINED FROM DEDIFFERENTIATED REPROGRAMMED CELLS | 2014 |
|
RU2754089C2 |
| SC-β CELLS AND COMPOSITIONS AND METHODS FOR THEIR CREATION | 2014 |
|
RU2813705C1 |
| SC-β CELLS AND COMPOSITIONS AND METHODS FOR THEIR CREATION | 2014 |
|
RU2772585C2 |
| SC-β CELLS AND COMPOSITIONS AND METHODS FOR THEIR CREATION | 2014 |
|
RU2692595C2 |
| INDUCING PANCREATIC BETA CELLS BY STEM CELL DIFFERENTIATION UNDER ACTION OF RNA | 2017 |
|
RU2822203C2 |
