FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to methods for differentiating PDX1-positive pancreatic precursor cells to NKX6.1-positive pancreatic precursor cells using a culture medium. In this case, the culture medium contains KGF, SANT1 and retinoic acid (RA) and does not contain a protein kinase C (PKC) activator or does not contain an inhibitor of BMP signal path.
EFFECT: obtaining SC-β cells and compositions.
11 cl, 17 dwg, 6 tbl, 1 ex
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