METHOD FOR NON-INVASIVE DIAGNOSIS OF ENDOMETRIOSIS BY LIPID PROFILE OF DRY DROPS OF MENSTRUAL BLOOD Russian patent published in 2024 - IPC G01N33/92 G01N30/72 G01N33/49 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to gynaecology, and can be used for diagnosing endometriosis. Samples of patient’s menstrual blood are obtained, lipid extract of dry drops of menstrual blood is prepared, lipid profile of dry menstrual blood spots is obtained by high-performance liquid chromatography with mass spectrometry (HPLC-MS). Based on lipids PE P-16:0/18:1 and PE P-18:0/20:4, calculated is a response variable y':

where I_{PE P-16:0/18:1} ^_ area of peaks of lipid marker of plasmenyl-phosphatyl ethanolamine PE P-16:0/18:1 on a chromatogram of a sample of a lipid extract of a dry spot of menstrual blood of a patient, standard units; I_{PE P-18:0/20:4} ^_ area of peaks of the lipid marker of plasmenyl-phosphatyl ethanolamine PE P-18:0/20:4 on a chromatogram of a sample of a lipid extract of a dry spot of menstrual blood of a patient, standard units. At value y' from 0.68 to 1, endometriosis is diagnosed in the patient. At value y' from 0 to 0.68, the absence of endometriosis is diagnosed in the patient.

EFFECT: method enables early non-invasive diagnosis of endometriosis of various severity by analysing the lipid profile of dry drops of menstrual blood by HPLC-MS as a result of applying a classifying model built on the basis of a logistic regression method for calculating a response variable (y'): taking into account levels of 2 lipids: PE P-16:0/18:1 and PE P-18:0/20:4.

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