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2 828 869

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IPC

C12Q1/6886(2018-01-01)

(21) (22)

Application

2024115052, 2024-06-03

(24)

Start date

2024-06-03

(22)

Actual filing date

2024-06-03

(45)

Published

2024-10-21

(72)

Inventor

Bushueva Olga IurevnaKobzeva Kseniia Andreevna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennyi Meditsinskii Universitet" Ministerstva Zdravookhraneniia Rossiiskoi Federatsii

METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs1043618 (G>C) OF HSPA1A GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES Russian patent published in 2024 - IPC C12Q1/6886

Abstract RU 2828869 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics and can be used in medicine for determining hereditary predisposition to developing diseases associated with carriage of polymorphic variant rs1043618 (G>C) of HSPA1A gene. Disclosed is a method for genotyping the polymorphic rs1043618 (G>C) HSPA1A gene in a human by real-time PCR using allele-specific fluorescent probes, involving PCR using specially selected primers (forward 5'-ATCCAGTGTTCCGTTTCCAG-3' and reverse 5'-GAGTAGGTGGTGCCCAGGT-3') and probes with fluorophores (G-allele-specific fluorescent-labelled probe 5'-(FAM)CTCAGAGCGGAGCCGAC(RTQ1)-3' and C-allele-specific fluorescent-labelled probe 5'-(ROX)CTCAGAGCCGAGCCGAC(BHQ2)-3') in an amplifier with fluorescence detection.

EFFECT: invention widens the range of methods for genotyping polymorphic variants of the HSPA1A gene, is characterized by simplicity, accuracy and low cost.

1 cl, 1 dwg

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RU 2 828 869 C1

Authors

Bushueva Olga Iurevna

Kobzeva Kseniia Andreevna

Dates

2024-10-21—Published

2024-06-03—Filed