FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, molecular-genetic diagnostics and can be used in medicine in determining the hereditary predisposition to the development of diseases associated with the carriage of the polymorphic variant rs196336 (T>C) of the BAG3 gene. Disclosed is a method for genotyping polymorphic rs196336 (T>C) of the BAG3 gene in a human by real-time PCR using allele-specific fluorescent probes, which involves PCR using specially selected primers (forward 5'-CACCTGCAGTCTTCCTGGAT-3' and reverse 5'-CATCCGGCCTCTGTTTATTC-3') and probes with fluorophores (T-allele-specific fluorescent-labelled probe 5'-(FAM)CAGGCCCTTTCCTGGC(RTQ1)-3' and C-allele-specific fluorescent-labelled probe 5'-(ROX)CAGGCCCCTTCCTGGC(BHQ2)-3') in an amplifier with fluorescence detection.
EFFECT: invention widens the range of methods for genotyping polymorphic variants of the BAG3 gene, is characterized by simplicity, accuracy and low cost.
1 cl, 1 dwg
