FIELD: medicine.
SUBSTANCE: invention relates to medicine, namely to urology, andrology, reproductology, clinical laboratory diagnostics, and can be used to determine the need for assisted reproductive technology (ART) programs with sperm donation in males with obstructive azoospermia. For this purpose, testicular biopsy material is obtained in males with reproductive tract obstruction. Then, spermatogenesis state is examined by light microscopy on a histological section of cross sections of convoluted seminiferous tubules on a ten-point scale. Further, testicular interstitium and presence of tryptase-positive mast cells in 1 mm2, which are contrasted by means of immunohistochemical staining, are examined. If the value of tryptase-positive mast cells is 23 and more in 1 mm2 in the interstitium of the testicle with underlying spermatogenesis disorder of 6 points and below, the need to perform a protocol of assisted reproductive technologies with sperm donation is determined.
EFFECT: invention enables to determine the success of ART programs in obstructive azoospermia, thus to take timely measures for implementation of repeated protocol of assisted reproductive technologies using autospermia or donation of germ cells, thereby reducing the number of unsuccessful attempts of ART.
1 cl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETERMINING NEED FOR ASSISTED REPRODUCTIVE TECHNOLOGIES (ART) WITH SPERM DONATION IN IDIOPATHIC MALE INFERTILITY WITH NON-OBSTRUCTIVE AZOOSPERMIA | 2023 |
|
RU2814377C1 |
METHOD FOR PREDICTION OF PRESENCE OF SPERM CELLS IN HUMAN TESTICULAR TISSUE IN BIOPSY IN PATIENTS WITH AZOOSPERMIA USING RAMAN SPECTROSCOPY OF SEMINAL PLASMA | 2024 |
|
RU2829263C1 |
METHOD FOR ISOLATING SPERMATOZOA FROM ASPIRATION AND/OR BIOPSY MATERIAL FROM AN APPENDAGE AND/OR TESTICLE FOR USE IN IN VITRO FERTILIZATION AND/OR CRYOPRESERVATION PROGRAMS | 2021 |
|
RU2762489C1 |
METHOD FOR PREDICTING SPERMATOGENESIS STIMULATION EFFECTIVENESS IN OLIGOSPERMIC INFERTILITY (SECRETORY OLYGO- AND AZOOSPERMIA) CASES | 2004 |
|
RU2302641C2 |
METHOD FOR DETERMINING INDICATIONS FOR OPEN TESTICULAR BIOPSY IN PATIENTS WITH AZOOSPERMIA | 2017 |
|
RU2655776C1 |
METHOD OF PROTECTION AND RECOVERY OF SPERMATOGENESIS IN SURGICAL INTERVENTIONS ON THE SPERMATIC CORD, TESTIS, EPIDIDYMIS | 2020 |
|
RU2735888C2 |
METHOD OF DIAGNOSING REPRODUCTIVE FUNCTION IN MEN | 2009 |
|
RU2419097C1 |
METHOD OF CYTOLOGICAL DIAGNOSTICS OF SPEMATOGENESIS DISORDERS | 2007 |
|
RU2328736C1 |
METHOD OF TREATING MALE INFERTILITY | 2020 |
|
RU2738543C2 |
METHOD OF MINIMALLY INVASIVE ISOLATION OF BACTERIAL DNA FROM TESTICULAR TISSUE BIOPSY IN INFERTILE MEN | 2022 |
|
RU2810467C1 |
Authors
Dates
2024-11-26—Published
2024-05-22—Filed