FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for detecting antibodies to recombinant proteins VP1, VP2 and VP3 Echovirus 30. Said method involves an enzyme immunoassay for IgA-, IgM- and IgG-antibodies in the presence of recombinant antigens, which are surface proteins VP1, VP2 and VP3 Echovirus 30, wherein the presence of antibodies to each of the VP1, VP2 or VP3 proteins is determined by the increase in the optical density of the reaction relative to the control - bovine serum in dilution of 1:10.
EFFECT: present invention provides detection of specific antibodies to recombinant proteins VP1, VP2 and VP3 of enterovirus Echovirus 30 by enzyme immunoassay.
5 cl, 5 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR OBTAINING VIRUS-LIKE PARTICLES OF NOROVIRUS CONTAINING THE PROTEIN VP1 OF ENTEROVIRUS ECHOVIRUS 30, IN VITRO | 2022 |
|
RU2789354C1 |
| STRAIN OF ENTEROVIRUS COXSACKIE B6 SELECTIVELY INFECTING AND LYSING TUMOROUS HUMAN CELLS in vitro | 2012 |
|
RU2496873C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR OBTAINING FULL NUCLEOTIDE SEQUENCE OF VP1 GENE OF COXSACKIE A10 ENTEROVIRUS | 2020 |
|
RU2762236C1 |
| METHOD OF EARLY POSTOPERATIVE COMPLICATIONS PREDICTION IN PATIENTS WITH RHEUMATIC HEART DISEASES | 2006 |
|
RU2336528C2 |
| METHOD FOR DIFFERENTIAL AMPLIFICATION OF VP1 REGION FRAGMENT OF ENTEROVIRUS GENOME ENTEROVIRUS A AND ENTEROVIRUS B | 2020 |
|
RU2743352C1 |
| METHOD OF DETECTION AND DIFFERENTIATION OF ENTEROVIRUS RNA | 2000 |
|
RU2189396C2 |
| METHOD FOR ENTEROVIRUS GENOTYPING BY GENOME REGION 1A-1B SEQUENCING METHOD | 2019 |
|
RU2701145C1 |
| METHOD FOR AMPLIFYING THE VP1 REGION OF THE GENOME OF ENTEROVIRUS SPECIES ENTEROVIRUS B | 2021 |
|
RU2774424C1 |
| METHOD FOR OBTAINING ACTIVE STARTING OF VACCINE AGAINST ROTAVIRUS INFECTION OF PROTEIN FLiCVP6VP8 | 2017 |
|
RU2649132C1 |
| METHOD FOR CARRYING OUT AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES IN A HUMAN BIOLOGICAL SAMPLE SPECIFIC TO THE SARS-COV2 HUMAN CORONAVIRUS, A TEST SYSTEM | 2021 |
|
RU2759149C1 |
