FIELD: biotechnology, microbiology. SUBSTANCE: method involves stages of fermentation of indicated xylose an aqueous solution using yeast strain showing capability to convert free xylose to xylitol and free hexoses in indicated solution to ethanol for time sufficient for preparing the fermentation solution containing xylitol. Method involves also separation of one or some xylitol-enriched fractions and indicated fermentation solution by chromatorgaphy. EFFECT: improved method of preparing. 23 cl, 4 dwg, 4 tbl
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