FIELD: molecular biology, genetic engineering, biochemistry. SUBSTANCE: invention relates to polypeptide of molecular mass 38 700 Da showing activity of carminomycin-4-O-methyltransferase. Polypeptide is obtained by culturing strain-producers Escherichia coli or Streptomyces. Strains are transformed with recombinant plasmids pNHM 901 or pWHM 902 or pWHM 903 that contain DNA fragment of the identified nucleotide sequence. EFFECT: improved method of polypeptide preparing. 3 cl, 7 dwg
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