FIELD: genetic engineering, antibiotics. SUBSTANCE: as result of screening the genome library of the strain Streptomyces peucetius (ATCC 29050) DNA fragment is cloned that comprises gene encoding daunorubicin 14-hydroxylase. By transformation of strains Streptomyces and E. coli with vector containing the indicated gene recombinant strains exhibiting capacity to convert daunorubicin to doxorubicin effectively in culturing have been obtained. Invention can be used for industrial preparing antibiotic doxorubicin eliciting an antitumor activity. EFFECT: improved preparing method of doxorubicin. 6 cl, 3 dwg
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