FIELD: veterinary microbiology, biotechnology. SUBSTANCE: industrial strains of Salmonella choleraesuis and Salmonella typhimurium are cultured in nutrient medium containing Hottinger broth at pH 7.6-7.8 and index of amine nitrogen 200-250 mg%. Obtained bacterial mixture is subjected for inactivation with aminoethylethyleneimine which is added to bacterial suspension up to concentration 0.5-4%. Mixture is incubated at 37-38 C for 12-16 h. After termination of inactivation obtained antigen is added to sucrose-gelatin medium and antigen is combined with oily adjuvant in the ratio = (2: 3)-(1: 4), respectively. EFFECT: enhanced immunogenic activity and specific safety of vaccine. 5 cl, 3 tbl, 6 ex
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Authors
Dates
2001-01-27—Published
2000-04-17—Filed