METHOD FOR PREPARING COMPLEXLY METHYLATED DNA FINGERPRINTS Russian patent published in 2004 - IPC

FIELD: molecular biology, biochemistry, medicine, oncology. SUBSTANCE: invention relates to method for characterization, classification and differentiation of tissues and cell types, prediction of behavior of tissues and cell groups and identification of genes with alternated expression. Method involves conversion of cytosine base (but not 5-methylcytosine) in genome DNA prepared from any tissue sample to uracil by treatment with bisulfite solution and the following amplification of treated genome DNA fraction using very short or degenerated oligonucleotides. Remained cytosines of amplified fractions are determined by hybridization method or using polymerase chain reaction. Method represents the novel possibility for carrying out the differential diagnosis of cancer diseases. EFFECT: improved prepared method, valuable biological and medicinal properties. 19 cl, 8 dwg, 5 tbl, 2 ex