METHODS OF DETECTING MODIFICATION OF NUCLEOTIDES Russian patent published in 2017 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology, namely to methods of identifying modified cytosine residues innucleotide sequence. Population of polynucleotides is provided, which contain analyzed nucleotide sequence. First part of above population is oxidized or restored, then it is treated with bisulphite. Polynucleotides are sequenced in first and second parts of population. Residue corresponding to cytosine residue in investigated nucleotide sequence is identifying in first and second nucleotide sequences. In particular version method of identifying 5-methylcytosine in investigated nucleotide sequence is proposed. Population of polynucleotides is oxidized, it is treated with bisulphite, sequenced and residue is identified in treated nucleotide sequence. Presence of cytosine in treated nucleotide sequence indicates presence of 5-methylcytosine in investigated nucleotide sequence. Inventions enable to distinguish residues of 5-methylcytosine, 5-hydroxymethylcytosine and 5-formilcitozin from cytosine at mononucleotide resolution.

EFFECT: methods are applicable to all sequencing platforms and can be used in analysis of genomic DNA and/or RNA.

27 cl, 5 tbl, 11 dwg