METHOD FOR DETERMINATION OF FLUORESCEIN SYNTHESIS WITH Pseudomonas BACTERIA Russian patent published in 2009 - IPC C12Q1/00 C12N1/20 C12R1/38 

FIELD: medicine.

SUBSTANCE: invention can be used in bacteriological tests for identification of bacteria Pseudomonas and manufacturing of nutrient mediums for these tests. The method involves preparation of the nutrient medium by dissolution of L-glutamine or L-glutamic acid (L-glutamate sodium), NaCl, Na₂SO₄, KH₂PO₄, K₂HPO₄, MgSO₄·7H₂O, agar in specified amount when boiled in 1 litre of distilled water. Glycerol in specified amount is added. The prepared nutrient medium is boiled within 5 minutes. Herewith, medium pH is 7.0±0.2. It is followed with inoculation of day culture Pseudomonas in said nutrient medium. The inoculations are incubated within 24-48 hours at temperature 28°C, then irradiated with UV light and analysed for fluorescein considering yellow-green fluorescence of bacterial lawn and surrounding zone of the nutrient medium.

EFFECT: simplified method for preparing the nutrient medium herewith preserving its nutritious properties and increasing commonality characteristics.

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