FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to microbiology. Day old agar bacterial cultures are inoculated in a single loop in a sodium chloride solution on a sector of culture medium, which contains barium ions of barium chloride, and the same culture medium without barium ions (control). Seeds are incubated at 28°C for 24 hours, after which Pseudomonas bacteria is identified from lack of growth of bacteria on the culture medium with barium ions and growth of bacteria on the same culture medium without barium ions. Presence of yellow-green fluorescence of bacterial lawn and the culture medium surrounding it indicates a group of fluorescent pseudomonade. Synthetic culture medium is used with the following content of ingredients, g/l: L-proline 1.0 to 3.0, L-sodium glutamate or L-glutamine 1.0 to 3.0, NaCl 5.0, MgCl2•6H2O 0.05, KH2PO4 0.05, K2HPO4 0.1, bacteriological agar 15.0, distilled water up to 1 litre, pH - 7.2+0.2, barium chloride supplement (BaCl2•2H2O) 6.0 to 16.0. The ingredients, except barium chloride, are dissolved while heating. The medium is boiled for 5 minutes. Part of the ready medium is poured into sterile Petri dishes (culture medium without barium ions). An amount of barium chloride calculated using a given formula is added to the remaining part of hot medium. The mixture is stirred and poured into sterile dishes (culture medium with barium ions).
EFFECT: invention increases standardness and simplifies identification of Pseudomonas bacteria.
2 tbl, 4 ex
