FIELD: medicine.
SUBSTANCE: method provides DNA recovery from biological material samples, polymerase chain reaction with recovered DNA and amplification product detection. Target amplicon detection as PCR products testifies to the presence of Cryphonectria parasitica in the analysed sample. DNA recovery from vegetable tissue samples is ensured by using lytic buffers containing antioxidants, while the PCR requires applying the primers specific to low-polymorphous sequences of precursor protein genes of C.parasitica sex pheromones.
EFFECT: invention allows recognising efficiently a pathogen and can be applied for quick diagnosing of chestnut blight.
2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF ANIMAL DNA IN FORAGE AND MEAT PRODUCTS | 2011 |
|
RU2560579C2 |
| METHOD FOR PCR IDENTIFICATION OF PHYTOPATHOGENIC FUNGUS FUSARIUM OXYSPORUM | 2022 |
|
RU2816852C1 |
| METHOD FOR PCR IDENTIFICATION OF PHYTOPATHOGENIC FUNGUS ALTERNARIA SOLANI | 2022 |
|
RU2804687C1 |
| METHOD FOR DNA ISOLATION FROM PLANTS, SUITABLE FOR PCR | 2017 |
|
RU2672378C1 |
| METHOD FOR SPECIFIC DETECTION OF Trichophyton mentagrophytes IN CLINICAL MATERIAL IN VARIOUS CLINICAL FORMS OF DISEASES | 2014 |
|
RU2563619C1 |
| METHOD FOR MULTIPLEX PCR DETECTION OF Atopobium vaginae, Leptotrichia amnionii, Sneathia sanguinegens AND Eggerthella spp. IN CLINICAL MATERIAL | 2015 |
|
RU2583924C1 |
| METHOD TO DETECT GENES THAT DETERMINE ADHESINS, HEMOLYSINS, MANNOSE-RESISTANT HAEMAGGLUTININS IN GENITAL STRAINS OF Escherichia coli | 2012 |
|
RU2483114C1 |
| METHOD OF SPECIFIC DETECTION OF TRICHOPHYTON VERRUCOSUM IN CLINICAL MATERIAL IN CASE OF DIFFERENT CLINICAL FORMS OF DISEASE | 2014 |
|
RU2562540C1 |
| METHOD FOR SPECIFIC DETECTION OF MICROSPORUM CANIS IN CLINICAL MATERIAL IN VARIOUS CLINICAL FORMS OF DISEASE | 2014 |
|
RU2558927C1 |
| MICROBIAL TEST STRAIN OF Helicobacter pylori GKPM-Obolensk V-7215 FOR INDICATION AND IDENTIFICATION OF Helicobacter pylori IN SAMPLES OF BIOPSY MATERIALS OF GASTRIC OR DUODENAL ULCER | 2015 |
|
RU2595422C1 |
