CATIONIC OLIGONUCLEOTIDES, AUTOMATED SYNTHESIS METHODS THEREOF AND USE THEREOF Russian patent published in 2012 - IPC C07H21/00 

FIELD: chemistry.

SUBSTANCE: invention relates to oligonucleotide-oligocationic molecules A_iB_jH, which are used in molecular biology, diagnosis and therapeutic versions of use. The oligonucleotide-oligocationic molecules A_iB_jH can be synthesised via automated phosphorus amidite chemistry and have oligonucleotide fragments A_i and oligocationic fragments B_j. The fragment A_i is an i-dimensional oligonucleotide residue with index i from 5 to 50, in which nucleotide A is an oligomer with natural or synthetic nucleotide bases and/or pentafuranosyl groups and/or native phosphodiester bonds, as well as chemical modifications or substitutions thereof. Fragment B_j is a j-dimensional organic oligocationic fragment with index j from 1 to 50, in which B is selected from a group comprising -HPO₃-R¹-(X-R² _n)_n1-X-R³-O-, where R¹, R² _n and R³, identical or different, denote C1-C5 alkylene, X denotes NH or NC(NH₂)₂, index n1 = 2 to 20; -HPO₃-R⁴-CH(R⁵X¹)-R⁶-O-, where R⁴ denotes C1-C5 alkylene, R⁵ and R⁶, identical or different, denote C1-C5 alkylene, and X¹ denotes a putrescine, spermidine or spermine residue.

EFFECT: high efficiency of the method.

18 cl, 14 dwg, 5 ex