FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to the field of biotechnology. Claimed is a method of specific isolation of the complete DNA-content of bacterial pathogens. Non-viable cells are separated from viable ones by centrifugation. A washing solution, containing a detergent and an electrolyte, is used. Lysis of the viable cells is carried out. Lysate DNA is connected with a matrix, washed and eluted.
EFFECT: high indices of measurement technology, high output of bacterial DNA, method sensitivity and rapidity of the method implementation are achieved.
4 cl, 6 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD AND KIT FOR DETECTION OF MICROORGANISMS | 2010 |
|
RU2527897C2 |
| METHOD FOR ISOLATING OF COCCIDIOIDES IMMITIS DNA FOR POLYMERASE CHAIN REACTION (PCR) | 2005 |
|
RU2295569C1 |
| UNIVERSAL METHOD OF DNA ISOLATION AND LYSIS MIXTURE FOR ITS IMPLEMENTATION | 2022 |
|
RU2807254C1 |
| ANALYSIS SYSTEM FOR ORTHOGONAL ACCESS TO BIOMOLECULES AND THEIR LABELLING IN CELL COMPARTMENTS | 2017 |
|
RU2771892C2 |
| REPLACEABLE MICROFLUID MODULE FOR AUTOMATED RECOVERY AND PURIFICATION OF NUCLEIC ACIDS FROM BIOLOGICAL SAMPLES AND METHOD FOR RECOVERY AND PURIFICATION NUCLEIC ACIDS WITH USING THEREOF | 2008 |
|
RU2380418C1 |
| METHODS FOR DISCRETE AMPLIFICATION OF COMPLETE GENOME | 2016 |
|
RU2736351C2 |
| AUTOMATED DEVICE FOR ISOLATION, PURIFICATION AND ANALYSIS OF NUCLEIC ACIDS BY RT-PCR METHOD | 2020 |
|
RU2784821C2 |
| TEST TUBE FOR BEADS IMPACT TREATMENT AND A METHOD OF EXTRACTING DISOROBONUCLEIC ACID AND/OR RIBONUCLEIC ACID FROM MICROORGANISMS | 2017 |
|
RU2743140C2 |
| METHODS FOR DNA AMPLIFICATION TO PRESERVE METHYLATION STATUS | 2018 |
|
RU2754038C2 |
| TEST TUBE FOR IMPACT PROCESSING WITH BALLS AND METHOD FOR EXTRACTION OF DEOXYRIBONUCLEIC ACID AND/OR RIBONUCLEIC ACID FROM MICROORGANISMS | 2018 |
|
RU2762314C2 |
