FIELD: medicine.
SUBSTANCE: method refers to virology and concerns a method for viral polyhedron decontamination. The invention involves homogenising dead insect biomass, centrifuging and precipitating. A viral polyhedron suspension is pre-shaken and centrifuged until a white fraction of viral polyhedrons appears above precipitated insect tissues. A supernatant with polyhedrons is removed into a new test tube and added with a three-molar guanidine thiocyanate solution, centrifuged once again; the supernatant is removed; the test tube is added with water and re-suspended on a centrifuge. Precipitated polyhedrons are added with TE buffer, kept for 180 s at temperature of +25 degrees Celsius, centrifuged; the supernatant is removed; the precipitate contains decontaminated polyhedron viruses.
EFFECT: invention is applicable in an entomopathogenic-virus insecticide technology as an intermediate stage of virus recovery from dead insects.
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