FIELD: biotechnologies.
SUBSTANCE: recombinant DNA is offered, which provides in the composition of a plasmid vector the synthesis in cells of E.coli of a chimeric protein - apolipoprotein A-I of a human being, with the size of 852 bps. The recombinant DNA in series includes from 5'- end DNA fragment coding 6 a.o. of histidine with the size of 18 bps (SEQ ID NO 1), DNA fragment coding 6 a.o. pro-form of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding N-end fragment from 15 a.o. of bull pancreatic RNAase A (SEQ ID NO 3) with the size of 45 bps, DNA fragment coding 6 a.o. pro-form- of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding the site of the recognition of protease of a tobacco mosaic virus with the size of 21 bps (SEQ ID NO 4), mutant gene of a mature protein apoA-I of a human being with the size of 729 bps (SEQ ID NO 5). The yield of the chimeric protein of apolipoprotein A-I of a human being from 1 l of the culture of the cells E.coli is 182±32 mg that amounts 39.7±6.2 mg from 1 g of the biomass.
EFFECT: veracity improvement.
5 dwg, 3 tbl, 9 ex
Title |
Year |
Author |
Number |
RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I FOR PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I, Pichia pastoris YEAST STRAIN - PRODUCER OF RECOMBINANT CHIMERIC PROTEIN SUMO3-apoA-I AND METHOD OF PRODUCING MATURE HUMAN APOLIPOPROTEIN A-I |
2015 |
- Mamaev Aleksej Lvovich
- Beklemishev Anatolij Borisovich
|
RU2605326C1 |
RECOMBINANT CHIMERIC POLYPEPTIDES, CARRYING EPITOPES OF VARIOUS IMMUNODOMINANT PROTEINS OF SPIROCHAETA OF BORRELIA BURGDORFERI SENSU LATO COMPLEX, AND SERUM DIAGNOSTIC TECHNIQUE FOR IXODIC TICK-BORNE BORRELIOSIS |
2012 |
- Beklemishev Anatolij Borisovich
- Rjabchenko Aleksandr Vladimirovich
- Karavaev Vitalij Semenovich
|
RU2514230C1 |
ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN |
2012 |
- Ramazanov Jurij Akhmetovich
- Beklemishev Anatolij Borisovich
|
RU2512527C2 |
RECOMBINANT PLASMID VECTOR pET32-WNV-DIII, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT DOMAIN III OF STRUCTURAL GLYCOPROTEIN E OF WEST NILE VIRUS IN ESCHERICHIA COLI CELLS, ESCHERICHIA COLI BL21(DE3)-DIII-WNV CELL LINE STRAIN AND DIII-WNV RECOMBINANT PROTEIN FOR PREPARING IMMUNOBIOLOGICAL PREPARATIONS |
2024 |
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Isaeva Anastasiya Aleksandrovna
- Volosnikova Ekaterina Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
|
RU2821341C1 |
RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN |
2020 |
- Beklemishev Anatolij Borisovich
- Pykhtina Mariya Borisovna
|
RU2764787C1 |
RECOMBINANT PLASMID DNA PSMT3_HCRG21 ENCODING A HYBRID PROTEIN SMT3-HCRG21, BACTERIAL STRAIN ESCHERICHIA COLI BL21(DE3)/PSMT3_HCRG21 - PRODUCER OF HCRG21 ANALGESIC PEPTIDE AND METHOD OF PRODUCING RECOMBINANT HCRG21 ANALGESIC PEPTIDE |
2022 |
- Tereshin Mikhail Nikolaevich
- Stepanenko Vasilii Nikolaevich
- Leichenko Elena Vladimirovna
- Kozlovskaia Emma Pavlovna
- Podvolotskaia Anna Borisovna
- Tekuteva Liudmila Aleksandrovna
- Kozlov Sergei Aleksandrovich
|
RU2798545C1 |
RECOMBINANT PROTEIN CONSTRUCT DSD-sp-β-GAL EXHIBITING ENZYMATIC ACTIVITY OF THERMOSTABLE β-GALACTOSIDASE (LACTASE) AND AFFINE CONNECTABLE WITH DEXTRANE, PLASMID DNA pGD-10 DETERMINING DSD-sp-β-GAL BIOSYNTHESIS, AND Escherichia coli DH5α/PGD-10 PRODUCER STRAIN |
2009 |
- Grishin Dmitrij Viktorovich
|
RU2428477C2 |
INTEGRAL PLASMID VECTOR pVEAL2-B7, PROVIDING EXPRESSION AND SECRETION OF RECOMBINANT PROTEIN B7 OF SMALLPOX VIRUS (SV) IN MAMMALIAN CELLS, RECOMBINANT STRAIN OF CELL LINE CHO-K1-B7 AND SV RECOMBINANT PROTEIN B7, PRODUCED BY STRAIN OF CELL LINE CHO-K1-B7 AND USED TO PRODUCE IMMUNOBIOLOGICAL PREPARATIONS |
2023 |
- Merkuleva Yuliya Aleksandrovna
- Nikitin Vladimir Nikolaevich
- Shcherbakov Dmitrij Nikolaevich
- Shanshin Daniil Vasilevich
- Sergeev Aleksandr Aleksandrovich
|
RU2817380C1 |
RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-7 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM |
2012 |
- Sharapova Natal'Ja Evgen'Evna
- Kotnova Alina Petrovna
- Sergienko Ol'Ga Vasil'Evna
- Galushkina Zoja Mikhajlovna
- Poletaeva Nina Nikolaevna
- Lavrova Natal'Ja Vital'Evna
- Grunina Tat'Jana Mikhajlovna
- Ljashchuk Aleksandr Mikhajlovich
- Semikhin Aleksandr Sergeevich
- Gromov Aleksandr Viktorovich
- Bartov Mikhail Sergeevich
- Subbotina Marina Evgen'Evna
- Karpova Tat'Jana Andreevna
- Ershova Anna Stepanovna
- Lunin Vladimir Glebovich
- Karjagina-Zhulina Anna Stanislavovna
- Gintsburg Aleksandr Leonidovich
|
RU2499047C1 |
RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-2 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM |
2012 |
- Sharapova Natal'Ja Evgen'Evna
- Kotnova Alina Petrovna
- Sergienko Ol'Ga Vasil'Evna
- Galushkina Zoja Mikhajlovna
- Poletaeva Nina Nikolaevna
- Lavrova Natal'Ja Vital'Evna
- Grunina Tat'Jana Mikhajlovna
- Ljashchuk Aleksandr Mikhajlovich
- Semikhin Aleksandr Sergeevich
- Gromov Aleksandr Viktorovich
- Bartov Mikhail Sergeevich
- Subbotina Marina Evgen'Evna
- Karpova Tat'Jana Andreevna
- Ershova Anna Stepanovna
- Lunin Vladimir Glebovich
- Karjagina-Zhulina Anna Stanislavovna
- Gintsburg Aleksandr Leonidovich
|
RU2499048C1 |