METHOD FOR ASSESSING ALLOGENIC IMMUNE RESPONSE IN SHORT-TERM MIXED MONONUCLEAR CULTURE OF UNRELATED DONORS Russian patent published in 2016 - IPC G01N33/49 

Abstract RU 2581925 C2

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for evaluation of Allogenic immune response in short-term mixed mononuclear culture of unrelated donors. For this purpose, with help of flow cytometry quick analysis is carried out with phenotype CD mononuclear b-lymphocyte subpopulation CD3+HLA-DR+, CD3-HLA-DR+, CD45+HLA-DR+ each donor, separated and gated by different fluorescent dye in mixed culture. Followed by calculating gain coefficients for each subpopulation. At positive values of CPCD3-HLA-DR+, and CPCD45+HLA-DR+ presence of immune according alloHLA factor CPCD3+HLA-DR+ indicates features of interacting genotypes HLA-DRB1*.

EFFECT: invention enables assessing immune reaction and detecting allogeneic cell sensitisation at allogenic antigens main complex tissue compatibility.

1 cl, 1 tbl, 2 dwg, 3 ex

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RU 2 581 925 C2

Authors

Chistjakova Guzel Nukhovna

Shabaldin Andrej Vladimirovich

Belenkova Olga Viktorovna

Mozes Vadim Gelevich

Matveeva Vera Gennadevna

Shabaldina Elena Viktorovna

Remizova Irina Ivanovna

Gazieva Irina Aleksandrovna

Dates

2016-04-20Published

2014-06-27Filed