FIELD: medicine.
SUBSTANCE: invention refers to medicine and can be used for evaluation of Allogenic immune response in short-term mixed mononuclear culture of unrelated donors. For this purpose, with help of flow cytometry quick analysis is carried out with phenotype CD mononuclear b-lymphocyte subpopulation CD3+HLA-DR+, CD3-HLA-DR+, CD45+HLA-DR+ each donor, separated and gated by different fluorescent dye in mixed culture. Followed by calculating gain coefficients for each subpopulation. At positive values of CPCD3-HLA-DR+, and CPCD45+HLA-DR+ presence of immune according alloHLA factor CPCD3+HLA-DR+ indicates features of interacting genotypes HLA-DRB1*.
EFFECT: invention enables assessing immune reaction and detecting allogeneic cell sensitisation at allogenic antigens main complex tissue compatibility.
1 cl, 1 tbl, 2 dwg, 3 ex
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