FIELD: medicine.
SUBSTANCE: invention can be used for early prediction of miscarriage. Method involves RNA recovery from epithelial cells of cervical canal in females, reverse transcription to produce mDNA, determination of CASP-3α expression (relative units) and COX-2 by quantitative polymerase chain reaction and calculation y1 and y2 by equations of linear discriminant function of following type:
y1=-0.8383+155.755*x1+27.2986*x2,
y2=-207.714+3,007.030*x1+533.609*x2,
where x1 is level of mRNA expression of CASP-3α (relative units), x2 is level of mRNA expression of COX-2 (relative units). Early miscarriage is predicted, if y1 is more y2. Development of pregnancy is predict if y2 is greater than y1. Presented invention enables effective prediction of miscarriage in 92.10 % cases.
EFFECT: method application enables timely implementation of required therapeutic and preventive actions for prevention of habitual miscarriage.
1 cl, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREDICTION OF A NON-DEVELOPING PREGNANCY | 2018 |
|
RU2689165C1 |
| METHOD FOR EARLY DIAGNOSIS OF RISK OF FETAL LOSS IN A MARRIED COUPLE IN THE PRESENCE OF UROGENITAL INFECTION IN MEN | 2017 |
|
RU2660557C1 |
| METHOD OF PREDICTING MISCARRIAGE IN THE FIRST TRIMESTER BY ASSESSING CYCLOOXYGENASE 2 AND PROSTAGLANDIN E2 IN THE PERIPHERAL BLOOD OF PREGNANT WOMEN DURING EXACERBATION OF CYTOMEGALOVIRUS INFECTION | 2023 |
|
RU2800080C1 |
| METHOD FOR DETERMINING PREDISPOSITION TO REPRODUCTIVE DISORDERS IN FEMALES IN CONDITIONS OF EXCESSIVE PHENOL CONTAMINATION | 2019 |
|
RU2719411C1 |
| PREDICTION OF PREMATURE BIRTH BASED ON GENE EXPRESSION PROFILE OF INNATE IMMUNITY IN CERVICAL CELLS | 2016 |
|
RU2620153C1 |
| METHOD FOR PREDICTING OF MISCARRIAGE OF INFECTIOUS GENESIS AT EARLY STAGES OF GESTATION | 2016 |
|
RU2616902C1 |
| METHOD FOR EARLY PREDICTION OF MISCARRIAGE ASSOCIATED WITH REACTIVATION OF CYTOMEGALOVIRUS INFECTION | 2022 |
|
RU2787451C1 |
| METHOD FOR PREDICTION OF PRETERM DELIVERY OF INFECTIOUS GENESIS | 2009 |
|
RU2408014C2 |
| METHOD OF PREDICTING MISCARRIAGE BY ASSESSING VASCULAR ENDOTHELIAL GROWTH FACTOR AND PROSTAGLANDIN F2Α IN THE PERIPHERAL BLOOD OF PREGNANT WOMEN DURING EXACERBATION OF CYTOMEGALOVIRUS INFECTION IN THE FIRST TRIMESTER | 2023 |
|
RU2800311C1 |
| METHOD FOR PREDICTING PRETERM BIRTH IN THE 2ND TRIMESTER OF PREGNANCY | 2022 |
|
RU2785050C1 |
